Inhibition of Germinal Vesicle Breakdown of Isolated Cumulus-Free Mouse Oocytes in Culture by a Calcium Inhibitor, TMB-8.
نویسندگان
چکیده
منابع مشابه
Inhibition of calcineurin by FK506 stimulates germinal vesicle breakdown of mouse oocytes in hypoxanthine-supplemented medium
Calcineurin (CN) is a serine/threonine phosphatase which plays important roles in meiosis maturation in invertebrate oocytes; however, the role of CN in mouse oocytes is relatively unexplored. In this study, we examined the expression, localization and functional roles of CN in mouse oocytes and granulosa cells. The RT-PCR results showed that the β isoform of calcineurin A subunit (Cn A) expres...
متن کاملthe effects of cumulus cells on in vitro maturation of mouse germinal vesicle stage oocytes
background: in vitro maturation (ivm) of oocytes is a promising technique to reduce the costs and avert the side-effects of gonadotropin stimulation for in vitro fertilization (ivf). the pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of ivm remains a challenge. objective: in this study, we investigated the effe...
متن کاملComparison of Different Vitrification Procedures on Developmental Competence of Mouse Germinal Vesicle Oocytes in the Presence or Absence of Cumulus Cells
Background An evaluation of the developmental competence of vitrified mouse germinal vesicle (GV) oocytes with various equilibration and vitrification times; in the presence or absence of cumulus cells and by comparison between the cryotop method and straws was performed. MaterialsAndMethods Mouse GV oocytes were considered in cumulus-denuded oocytes (CDOs) and cumulus-oocyte complexes (COCs) g...
متن کاملGerminal vesicle breakdown in the mouse oocyte.
Germinal vesicle breakdown in mouse oocytes in vivo and in vitro has been examined by electron microscopy. In vitro oocytes were studied immediately after release from follicles and at various times (05-11 h) in culture. Approximately 30 min after oocyte release, chromatin condensation begins along the convoluted nuclear envelope (NE). Chromatin granules are common in all condensing chromosomes...
متن کاملcyclic GMP Mediated Inhibition of Spontaneous Germinal Vesicle Breakdown Both with and without Cumulus in Mouse Oocyte
Intact germinal vesicle (GV) arrest and release are essential for maintaining the fertility of mammals inducing human. Intact germinal vesicle release, maturation of oocytes is maintained by very complex procedures along with folliculogenesis and is a critical step for embryonic development. Cyclic guanosine monophosphate (cGMP) has been suggested a key factor for meiotic arrest but so far its ...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 1992
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.54.605